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293t human kidney epithelial cells  (ATCC)


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    ATCC 293t human kidney epithelial cells
    (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. <t>293T</t> cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.
    293t Human Kidney Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38849 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    293t human kidney epithelial cells - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates"

    Article Title: Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates

    Journal: bioRxiv

    doi: 10.64898/2026.01.29.702607

    (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. 293T cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.
    Figure Legend Snippet: (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. 293T cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.

    Techniques Used: Transfection, Plasmid Preparation, Microscopy, Staining, Construct, Immunoprecipitation, Western Blot, Control, Positive Control, Cotransfection



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    (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. <t>293T</t> cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.
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    Image Search Results


    (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. 293T cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.

    Journal: bioRxiv

    Article Title: Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates

    doi: 10.64898/2026.01.29.702607

    Figure Lengend Snippet: (A) Confocal images of HeLa cells 24 h after transfection by mCherry-CycT1 with empty vector or with GFP-TAZ-WT. Images were taken using Zeiss LSM 710 confocal microscope. The nuclei were stained by DAPI. Bar , 10 µm. (B) Live-cell confocal time series of a representative HeLa cell coexpressing GFP-TAZ-WT and mCherry-CycT1, acquired over 32 s. Arrows mark puncta undergoing fusion. Bar , 4 µm. (C) CycT1 does not directly bind to TAZ. 293T cells transfected with the indicated constructs were subjected to immunoprecipitation with anti-HA (HA IP), followed by western blotting with anti-Flag and anti-HA antibodies. GAPDH serves as a loading control. While CycT1-F did not precipitate with HA-TAZ, a positive control showed co-precipitation of Flag-TAZ (F-TAZ) with HA-TAZ. (D) TAZ dosage controls CycT1 partitioning. HeLa cells were transfected with mCherry-CycT1 together with increasing amounts of GFP-TAZ-WT (0, 0.5, 1.5 µg). Bar , 4 µm. (E) CDK9 modulates the organization of TAZ-CycT1 condensates. HeLa cells were co-transfected with fixed amounts of mCherry-CycT1 (1.0 µg) and GFP-TAZ (1.0 µg) together with increasing amounts of CDK9-F (0, 0.5, 1.0 µg). The bottom row (no GFP-TAZ) is a control lacking GFP-TAZ, with co-transfection of mCherry-CycT1 (1.0 µg) and CDK9-F (1.0 µg). Bar , 4 µm. The images in panels B, D and E were acquired on a Zeiss LSM880 Live-Cell confocal microscope. All confocal images are representative of at least three independent experiments.

    Article Snippet: HeLa cells (cat. #CCL-2), MCF10A human mammary epithelial cells (cat. #CRL-10317), MDA-MB-231 human breast cancer epithelial cells (cat. #HTB-26) and 293T human kidney epithelial cells (cat. #CRL-3216) were from American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Transfection, Plasmid Preparation, Microscopy, Staining, Construct, Immunoprecipitation, Western Blot, Control, Positive Control, Cotransfection